| No. | Name | Pos. | Ht | Wt | Age | Exp. | College | Acquired |
| 2 | Mason Crosby | K | 6-1 | 207 | 25 | 3 | Colorado | D6c-07 |
| 7 | Jeremy Kapinos | P | 6-1 | 230 | 25 | 1 | Penn State | FA-08 |
| 10 | Matt Flynn | QB | 6-2 | 222 | 24 | 2 | Louisiana State | D7a-08 |
| 12 | Aaron Rodgers | QB | 6-2 | 220 | 25 | 5 | California | D1-05 |
| 16 | Brett Swain | WR | 6-0 | 203 | 24 | 1 | San Diego State | D7b-08 |
| 20 | Atari Bigby | S | 5-11 | 213 | 27 | 4 | Central Florida | FA-05 |
| 21 | Charles Woodson | CB | 6-1 | 202 | 32 | 12 | Michigan | UFA-06 (Oak.) |
| 24 | Jarrett Bush | CB/S | 6-0 | 200 | 25 | 4 | Utah State | W-06 (Car) |
| 25 | Ryan Grant | RB | 6-1 | 226 | 26 | 3 | Notre Dame | T-07 (NYG) |
| 27 | Will Blackmon | CB | 6-0 | 206 | 24 | 4 | Boston College | D4b-06 |
| 30 | John Kuhn | FB | 6-0 | 259 | 26 | 4 | Shippensburg | W-07 (Pit) |
| 31 | Al Harris | CB | 6-1 | 190 | 34 | 12 | Texas A&M-Kingsville | T-03 (Phil) |
| 32 | Brandon Jackson | RB | 5-10 | 220 | 23 | 3 | Nebraska | D2-07 |
| 33 | Brandon Underwood | CB | 6-1 | 198 | 23 | R | Cincinnati | D6b-09 |
| 35 | Korey Hall | FB | 6-0 | 243 | 26 | 3 | Boise State | D6a-07 |
| 36 | Nick Collins | S | 5-11 | 207 | 26 | 5 | Bethune-Cookman | D2a-05 |
| 37 | Aaron Rouse | S | 6-4 | 223 | 25 | 3 | Virginia Tech | D3b-07 |
| 38 | Tramon Williams | CB | 5-11 | 191 | 26 | 3 | Louisiana Tech | FA-06 |
| 41 | Spencer Havner | TE/LB | 6-3 | 248 | 26 | 1 | UCLA | FA-08 |
| 42 | DeShawn Wynn | RB | 5-10 | 238 | 25 | 3 | Florida | D7a-07 |
| 45 | Quinn Johnson | FB | 6-1 | 250 | 22 | R | Louisiana State | D5a-09 |
| 50 | A.J. Hawk | LB | 6-1 | 248 | 25 | 4 | Ohio State | D1-06 |
| 51 | Brady Poppinga | LB | 6-3 | 247 | 29 | 5 | Brigham Young | D4b-05 |
| 52 | Clay Matthews | LB | 6-3 | 245 | 23 | R | Southern California | D1b-09 |
| 54 | Brandon Chillar | LB | 6-3 | 243 | 26 | 6 | UCLA | UFA-08 (StL) |
| 55 | Desmond Bishop | LB | 6-2 | 238 | 25 | 3 | California | D6b-07 |
| 56 | Nick Barnett | LB | 6-2 | 236 | 28 | 7 | Oregon State | D1-03 |
| 59 | Brad Jones | LB | 6-3 | 232 | 23 | R | Colorado | D7-09 |
| 61 | Brett Goode | LS | 6-1 | 261 | 24 | 2 | Arkansas | FA-08 |
| 62 | Evan Dietrich-Smith | G/C | 6-2 | 305 | 23 | R | Idaho State | FA-09 |
| 63 | Scott Wells | C | 6-2 | 303 | 28 | 6 | Tennessee | FA-04 (D7-04) |
| 68 | Breno Giacomini | T | 6-7 | 311 | 23 | 2 | Louisville | D5-08 |
| 70 | T.J. Lang | G/T | 6-4 | 316 | 21 | R | Eastern Michigan | D4-09 |
| 71 | Josh Sitton | G | 6-3 | 317 | 23 | 2 | Central Florida | D4b-08 |
| 72 | Jason Spitz | C/G | 6-3 | 302 | 26 | 4 | Louisville | D3b-06 |
| 73 | Daryn Colledge | G/T | 6-4 | 308 | 27 | 4 | Boise State | D2a-06 |
| 74 | Aaron Kampman | LB | 6-4 | 265 | 29 | 8 | Iowa | D5a-02 |
| 76 | Chad Clifton | T | 6-5 | 320 | 33 | 10 | Tennessee | D2-00 |
| 77 | Cullen Jenkins | DE | 6-2 | 305 | 28 | 6 | Central Michigan | FA-04 |
| 78 | Allen Barbre | G/T | 6-4 | 305 | 25 | 3 | Missouri Southern State | D4-07 |
| 79 | Ryan Pickett | NT | 6-2 | 330 | 29 | 9 | Ohio State | UFA-06 (StL) |
| 80 | Donald Driver | WR | 6-0 | 194 | 34 | 11 | Alcorn State | D7b-99 |
| 85 | Greg Jennings | WR | 5-11 | 198 | 25 | 4 | Western Michigan | D2b-06 |
| 86 | Donald Lee | TE | 6-4 | 248 | 29 | 7 | Mississippi State | FA-05 |
| 87 | Jordy Nelson | WR | 6-3 | 217 | 24 | 2 | Kansas State | D2a-08 |
| 88 | Jermichael Finley | TE | 6-5 | 247 | 22 | 2 | Texas | D3-08 |
| 89 | James Jones | WR | 6-1 | 218 | 25 | 3 | San Jose State | D3a-07 |
| 90 | B.J. Raji | NT | 6-2 | 337 | 23 | R | Boston College | D1a-09 |
| 94 | Jarius Wynn | DE | 6-3 | 277 | 23 | R | Georgia | D6a-09 |
| 96 | Michael Montgomery | DE | 6-5 | 273 | 26 | 5 | Texas A&M | D6a-05 |
| 97 | Johnny Jolly | DE | 6-3 | 320 | 26 | 4 | Texas A&M | D6a-06 |
| 99 | Jeremy Thompson | LB | 6-4 | 270 | 23 | 2 | Wake Forest | D4a-08 |
| Derrick Martin | DB | 5-10 | 200 | 24 | 4 | Wyoming | T-09 (Balt) |
| Injured Reserve | |||||||||
| No. | Name | Pos. | Ht | Wt | Age | Exp. | College | Injury | Date On |
| 22 | Pat Lee | CB | 6-0 | 194 | 25 | 2 | Auburn | 09/05 | |
| 26 | Charlie Peprah | S | 5-11 | 203 | 26 | 4 | Alabama | 09/05 | |
| 44 | Evan Moore | TE | 6-6 | 247 | 24 | 2 | Stanford | 09/05 | |
| 46 | Devin Frischknecht | TE | 6-3 | 258 | 22 | R | Washington State | Leg | 08/25 |
| 91 | Justin Harrell | DE | 6-4 | 320 | 25 | 3 | Tennessee | 09/05 | |